Journal: Drug Design, Development and Therapy
Article Title: Regulatory Effects of Zhenxin Formula in Treating Doxorubicin-Induced Heart Failure: Network Pharmacology and Animal Experimental Verification
doi: 10.2147/DDDT.S513643
Figure Lengend Snippet: ZXF regulated the PI3K/PDK1/AKT/FoxO1 signaling pathway. (n=5). ( A ) Western blot of phosphorylated and total protein levels of PI3K, PDK1, AKT, and FOXO1. ( B – E ) Statistical results of p-PI3K/PI3K, p-PDK1/PDK1, p-AKT/AKT, and p-FOXO1/FOXO1. ** P <0.01 vs Con group; Δ P <0.05, ΔΔ P <0.01 vs Dox group.
Article Snippet: Anti-Caspase-3 antibody (D3R6Y), anti-AKT1 phospho (Thr308) antibody (4056S), anti-AKT1 antibody (2920S), anti-FOXO1 phospho (Ser256) antibody (9461T), anti-FOXO1 antibody (2880T), anti-PDK1 phospho (Ser241) antibody (3438T), and anti-PDK1 antibody (5662S) were purchased from CST.
Techniques: Western Blot